Flow Cytometer: The Need of Modern Hematology Laboratory

Authors

  • Harshita Dubey PhD Student, Lab Oncology, Institute Rotary Cancer Hospital (IRCH), All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, India
  • Amar Ranjan Additional Professor Lab Oncology, Institute Rotary Cancer Hospital (IRCH), All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, India
  • Mansi Modi MSc Medical Biochemistry Student, Jamia Humdard University, New Delhi, India
  • Rimlee Dutta Senior Resident Dept. of Pathology, AIIMS, Ansari Nagar, New Delhi, India
  • Lawan Ya Ranjan Master of Engineering (Management), RMIT University, Melbourne, Australia, India
  • Luxmi Devi PhD Student, Medical Oncology, IIRCH, AIIMS, Ansari Nagar, New Delhi, India
  • Smriti Khatri MPharm, Turacoz Healthcare Solutions, Gurugram, Haryana, India

Keywords:

Flow cytometer, hematology, immunohistochemistry

Abstract

Indeed, laboratory diagnosis is rapidly changing from what it was in the past to the present. Nowadays, flow cytometer (FCM) has become a novel introduction to the modern diagnostic technique, mainly in the field of hematology. In case of acute leukemia, peripheral blood, bone marrow examination, cytochemistry and immunohistochemistry for differentiation of myeloid or lymphoid lineage is required, which is feasible by flow cytometry. It has dramatically improved the diagnostic efficiency and reduced the duration of sampling along with better diagnostic outcomes as well as provided efficient therapeutic monitoring of any drug or drug regimen. It has also opened some more sensitive therapeutic plans, like monitoring “Minimal residual disease (MRD)”, which is not possible without FCM. Detection of MRD has led to improved overall survival of patients. It has also opened up huge opportunities for research, which has become an important part of academic curriculum nowadays. Considering the importance and absolute necessity for better outcomes in hematology, the knowledge of basic principle of FCM becomes indispensable. Here, we try to elucidate the elementary components of this technique and also highlight its uses. 

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Published

2022-07-10

Issue

Section

Review Article